Some laboratory scholars have proposed that the concept of “gene-free” must be used in clinical PCR detection and scientific research using gene amplification technology. How to ensure a “gene-free” clean space for PER testing, high-efficiency filtration and purification of the air in the biological safety cabinet may be a good choice. However, due to the small space in the cabinet, once pollution occurs, it is more difficult to remove. Therefore, attention should be paid to the correct operation of the vaporized hydrogen peroxide sterilizer and daily disinfection to maintain the biological safety fulcrum.
In the first step, the fan of the biological safety cabinet was operated for 30 minutes before and after the experiment. The high-efficiency filtration system of the biological safety cabinet can filter particles with a diameter of 0.3 microns up to 99%.
In the second step, spray 75% of the essence to make the bacterial protein coagulate and denature.
In the third step, UV light irradiation for 30 minutes can destroy the molecular structure of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) in microbial cells.
The combination of the three can be used as the daily disinfection and maintenance measures of the biological safety cabinet to eliminate the contaminated genetic material. In the absence of accidents such as specimen leakage, splashing, and biological hazard spillage, the disinfection effect can be guaranteed, and there are no corrosive and irritating shortcomings, and the operation is simple and easy to implement.
However, such daily disinfection and maintenance are often cumbersome and time-consuming, which brings great inconvenience to laboratory work. Is there a more convenient, efficient and verifiable biological safety cabinet sterilization method? The vaporized hydrogen peroxide sterilizer is different from most of the disinfection equipment on the market. It can be used with one hand and is specially designed for this kind of space disinfection of biological safety cabinets.