Usually, formaldehyde fumigation is used for sterilization in the clean area of ​​sterile pharmaceutical factories. However, as everyone pays more and more attention to the harmfulness of formaldehyde, many pharmaceutical companies have begun to replace formaldehyde with hydrogen peroxide sterilization. On the one hand, it avoids the problem of human safety, and on the other hand, it can be regarded as corporate welfare. Moreover, compared with many enterprises with relatively high production capacity, formaldehyde fumigation takes two to three days each time to discharge residues, and the hydrogen peroxide sterilization method can usually be completed within 2 to 6 hours from the start of sterilization to the safe entry of personnel. , the time saved can reduce the loss of a lot of production capacity. This paper focuses on the commonly used detection methods for the sterilization effect of hydrogen peroxide sterilization methods.

  1. Distribution of biological indicators: Bacillus stearothermophilus is usually used for vaporization sterilization, and Bacillus subtilis is usually used for sterilization in atomization equipment. Select log3, log6, etc. according to the sterilization level requirements of the user unit. After the sterilization, put the biological indicator into the culture medium and put it into a constant temperature incubator with a suitable culture temperature to observe the culture result.
  2. Auxiliary chemical indicator card: various types of hydrogen peroxide color development cards are arranged at various sterilization risk points or remote points in the sterilization area. The chemical indicator card will change color when it encounters hydrogen peroxide, and there are also chemical indicator cards. Can display different colors according to the concentration of hydrogen peroxide.
  3. Detection of planktonic bacteria, sedimentation bacteria, etc.

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